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Basic violet 3

Item number673126
CAS548-62-9
FormulaC25H30N3Cl
Molecular weight407,98 g/mol
Quantity 1X250MG
storage conditions20 °C
CoA / MSDSRetrieve CoA
Retrieve MSDS
Basic violet 3

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Basic violet 3

Basic violet 3

673126
548-62-9

1X250MG

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Safety Data Sheetaccording to Regulation (EC) No 1907/2006 (REACH)
Classifications according to Regulation (EC) No 1272/2008.
Printdate 20.12.2024
IDENTIFICATION OF THE SUBSTANCE/MIXTURE AND OF THE COMPANY/UNDERTAKING
Product name:Basic violet 3
Catalog No.:673126
Relevant identified uses of the substance or mixtureIdentified: Laboratory chemical
uses: R&D
Uses advised against:HPC Standards GmbH
Am Wieseneck 7

04451 Cunnersdorf
Deutschland


Tel. +49 34291 3372-36
Fax. +49 34291 3372-39
contact@hpc-standards.com
Emergency telephone numberHPC Standards Tel. +49 34291 3372-36
This number is only available during office hours.
HAZARDS IDENTIFICATION2.1 Classification of the substance or mixture
Classification according to Regulation (EC) No 1272/2008
Acute toxicity, Oral (Category 4), H302
Serious eye damage (Category 1), H318
Carcinogenicity (Category 2), H351
Acute aquatic toxicity (Category 1), H400
Chronic aquatic toxicity (Category 1), H410 Classification according to EU Directives 67/548/EEC or 1999/45/EC
R40
Xn Harmful R22
Xi Irritant R41
N Dangerous for the
environment
R50/53
Label elements
PictogramGHS05 acid
acid
GHS07 exclam
exclam
GHS08 silhouete
silhouete
GHS09 Aquatic-pollut-red
Aquatic-pollut-red
2.2 Label elements
Labelling according Regulation (EC) No 1272/2008 Pictogram Signal word Danger
Hazard statement(s)
H302 Harmful if swallowed.
H318 Causes serious eye damage.
H351 Suspected of causing cancer.
H410 Very toxic to aquatic life with long lasting effects.
Precautionary statement(s)
P273 Avoid release to the environment.
P280 Wear protective gloves/ eye protection/ face protection.
P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove
contact lenses, if present and easy to do. Continue rinsing.
P501 Dispose of contents/ container to an approved waste disposal plant.
Supplemental Hazard
Statements
none
2.3 Other hazards
This substance/mixture contains no components considered to be either persistent, bioaccumulative and
toxic (PBT), or very persistent and very bioaccumulative (vPvB) at levels of 0.1% or higher
COMPOSITION/INFORMATION ON INGREDIENTS3.1 Substances
Synonyms : Basic Violet 3
Methyl Violet 10B
Hexamethylpararosaniline chloride
Gentian Violet
Formula : C25H30ClN3
Molecular weight : 407,98 g/mol
CAS-No. : 548-62-9
EC-No. : 208-953-6
Index-No. : 612-204-00-2
Hazardous ingredients according to Regulation (EC) No 1272/2008
Component Classification Concentration
C.I. Basic violet 3 Included in the Candidate List of Substances of Very High Concern (SVHC) according
to Regulation (EC) No. 1907/2006 (REACH)
CAS-No.
EC-No.
Index-No.
548-62-9
208-953-6
612-204-00-2
Acute Tox. 4; Eye Dam. 1;
Carc. 2; Aquatic Acute 1;
Aquatic Chronic 1; H302,
H318, H351, H410
<= 100 %
Hazardous ingredients according to Directive 1999/45/EC
Component Classification Concentration
C.I. Basic violet 3 Included in the Candidate List of Substances of Very High Concern (SVHC) according
to Regulation (EC) No. 1907/2006 (REACH)
CAS-No.
EC-No.
Index-No.
548-62-9
208-953-6
612-204-00-2
Xn, N, Carc.Cat.3, R22 - R40 -
R41 - R50/53
<= 100 %
FormulaC25H30N3Cl
Molecular Weight (g/mol)407.98
CAS-No.548-62-9
FIRST AID MEASURES4.1 Description of first aid measures
General advice
Consult a physician. Show this safety data sheet to the doctor in attendance.
If inhaled
If breathed in, move person into fresh air. If not breathing, give artificial respiration. Consult a physician.
In case of skin contact
Wash off with soap and plenty of water. Consult a physician.
In case of eye contact
Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician.
If swallowed
Never give anything by mouth to an unconscious person. Rinse mouth with water. Consult a physician.
4.2 Most important symptoms and effects, both acute and delayed
The most important known symptoms and effects are described in the labelling (see section 2.2) and/or in
section 11
4.3 Indication of any immediate medical attention and special treatment needed
No data available
FIRE-FIGHTING MEASURES5.1 Extinguishing media
Suitable extinguishing media
Use water spray, alcohol-resistant foam, dry chemical or carbon dioxide.
5.2 Special hazards arising from the substance or mixture
Carbon oxides, Nitrogen oxides (NOx), Hydrogen chloride gas
5.3 Advice for firefighters
Wear self-contained breathing apparatus for firefighting if necessary.
5.4 Further information
No data available
ACCIDENTAL RELEASE MEASURES6.1 Personal precautions, protective equipment and emergency procedures
Use personal protective equipment. Avoid dust formation. Avoid breathing vapours, mist or gas. Ensure
adequate ventilation. Evacuate personnel to safe areas. Avoid breathing dust.
For personal protection see section 8.
6.2 Environmental precautions
Prevent further leakage or spillage if safe to do so. Do not let product enter drains. Discharge into the
environment must be avoided.
6.3 Methods and materials for containment and cleaning up
Pick up and arrange disposal without creating dust. Sweep up and shovel. Keep in suitable, closed
containers for disposal.
6.4 Reference to other sections
For disposal see section 13
HANDLING AND STORAGE7.1 Precautions for safe handling
Avoid contact with skin and eyes. Avoid formation of dust and aerosols.
Provide appropriate exhaust ventilation at places where dust is formed.
For precautions see section 2.2. 7.2 Conditions for safe storage, including any incompatibilities
Store in cool place. Keep container tightly closed in a dry and well-ventilated place.
Light sensitive.
Storage class (TRGS 510): Non Combustible Solids
7.3 Specific end use(s)
Apart from the uses mentioned in section 1.2 no other specific uses are stipulated
EXPOSURE CONTROLS/PERSONAL PROTECTION8.1 Control parameters
Components with workplace control parameters
8.2 Exposure controls
Appropriate engineering controls
Handle in accordance with good industrial hygiene and safety practice. Wash hands before breaks and
at the end of workday.
Personal protective equipment
Eye/face protection
Face shield and safety glasses Use equipment for eye protection tested and approved under
appropriate government standards such as NIOSH (US) or EN 166(EU).
Skin protection
Handle with gloves. Gloves must be inspected prior to use. Use proper glove removal technique
(without touching glove's outer surface) to avoid skin contact with this product. Dispose of
contaminated gloves after use in accordance with applicable laws and good laboratory practices.
Wash and dry Hands Body Protection
Complete suit protecting against chemicals, The type of protective equipment must be selected
according to the concentration and amount of the dangerous substance at the specific workplace.
Respiratory protection
Where risk assessment shows air-purifying respirators are appropriate use a full-face particle
respirator type N100 (US) or type P3 (EN 143) respirator cartridges as a backup to engineering
controls. If the respirator is the sole means of protection, use a full-face supplied air respirator. Use
respirators and components tested and approved under appropriate government standards such
as NIOSH (US) or CEN (EU). Control of environmental exposure
Prevent further leakage or spillage if safe to do so. Do not let product enter drains. Discharge into
the environment must be avoided.
PHYSICAL AND CHEMICAL PROPERTIES9.1 Information on basic physical and chemical properties
a) Appearance Form: powder
Colour: dark green
b) Odour No data available
c) Odour Threshold No data available
d) pH 2,5 - 3,5 at 10 g/l at 20 °C
e) Melting point/freezing
point
Melting point/range: 205 °C - dec.
f) Initial boiling point and
boiling range
No data available
g) Flash point No data available
h) Evaporation rate No data available
i) Flammability (solid, gas) No data available
j) Upper/lower
flammability or
explosive limits
No data available
k) Vapour pressure No data available
l) Vapour density No data available
m) Relative density 1,190 g/cm3 at 20 °C
n) Water solubility 50 g/l at 27 °C
o) Partition coefficient: noctanol/
water
log Pow: 1,172 at 25 °C
p) Auto-ignition
temperature
> 190 °C
q) Decomposition
temperature
No data available
r) Viscosity No data available
s) Explosive properties No data available
t) Oxidizing properties No data available 9.2 Other safety information
Bulk density 220 - 400 kg/m3
Surface tension 44,2 mN/m
STABILITY AND REACTIVITY10.1 Reactivity
No data available
10.2 Chemical stability
Stable under recommended storage conditions.
10.3 Possibility of hazardous reactions
No data available 10.4 Conditions to avoid
No data available
10.5 Incompatible materials
Strong oxidizing agents
10.6 Hazardous decomposition products
Other decomposition products - No data available
In the event of fire: see section 5
TOXICOLOGICAL INFORMATION11.1 Information on toxicological effects
Acute toxicity
LD50 Oral - Mouse - 96 mg/kg
LD50 Oral - Rabbit - 150 mg/kg
LD50 Intraperitoneal - Rat - 8,9 mg/kg
LD50 Intraperitoneal - Mouse - 5,1 mg/kg
LD50 Intraperitoneal - Rabbit - 5 mg/kg
LD50 Intraduodenal - Rabbit - 160 mg/kg
Skin corrosion/irritation
No data available
Serious eye damage/eye irritation
Severe eye irritation
Respiratory or skin sensitisation
No data available
Germ cell mutagenicity
Human
HeLa cell
DNA inhibition
Human
HeLa cell
Cytogenetic analysis
Human
lymphocyte
Cytogenetic analysis
Rat
Liver
DNA inhibition
Mouse
lymphocyte
DNA damage Hamster
ovary
Cytogenetic analysis
Mammal
lymphocyte
DNA damage
Mammal
Other cell types
Cytogenetic Analysis Non-mammalian
Other cell types
Cytogenetic analysis
Result: Equivocal evidence.
Histidine reversion (Ames)
Carcinogenicity
Limited evidence of a carcinogenic effect.
IARC: No component of this product present at levels greater than or equal to 0.1% is identified as
probable, possible or confirmed human carcinogen by IARC.
Reproductive toxicity
No data available
Specific target organ toxicity - single exposure
No data available
Specific target organ toxicity - repeated exposure
No data available
Aspiration hazard
No data available
Additional Information
RTECS: BO9000000
prolonged or repeated exposure can cause:, Nausea, Headache, Vomiting
ECOLOGICAL INFORMATION12.1 Toxicity
Toxicity to daphnia and
other aquatic
invertebrates
EC50 - Daphnia magna (Water flea) - 0,35 mg/l - 48 h
(OECD Test Guideline 202)
Toxicity to algae EC50 - Pseudokirchneriella subcapitata - 0,42 mg/l - 72 h
(OECD Test Guideline 201)
12.2 Persistence and degradability
Biodegradability Result: 10 % - Not readily biodegradable.
Ratio BOD/ThBOD 0,12 %
12.3 Bioaccumulative potential
No data available
12.4 Mobility in soil
No data available
12.5 Results of PBT and vPvB assessment
This substance/mixture contains no components considered to be either persistent, bioaccumulative and
toxic (PBT), or very persistent and very bioaccumulative (vPvB) at levels of 0.1% or higher.
12.6 Other adverse effects
Very toxic to aquatic life with long lasting effects
DISPOSAL CONSIDERATIONS13.1 Waste treatment methods
Product
Offer surplus and non-recyclable solutions to a licensed disposal company. Dissolve or mix the material
with a combustible solvent and burn in a chemical incinerator equipped with an afterburner and scrubber Contaminated packaging
Dispose of as unused product
TRANSPORT INFORMATION14.1 UN number
ADR/RID: 3077 IMDG: 3077 IATA: 3077
14.2 UN proper shipping name
ADR/RID: ENVIRONMENTALLY HAZARDOUS SUBSTANCE, SOLID, N.O.S. (C.I. Basic violet 3)
IMDG: ENVIRONMENTALLY HAZARDOUS SUBSTANCE, SOLID, N.O.S. (C.I. Basic violet 3)
IATA: Environmentally hazardous substance, solid, n.o.s. (C.I. Basic violet 3)
14.3 Transport hazard class(es)
ADR/RID: 9 IMDG: 9 IATA: 9
14.4 Packaging group
ADR/RID: III IMDG: III IATA: III
14.5 Environmental hazards
ADR/RID: yes IMDG Marine pollutant: yes IATA: yes
14.6 Special precautions for user
Further information
EHS-Mark required (ADR 2.2.9.1.10, IMDG code 2.10.3) for single packagings and combination
packagings containing inner packagings with Dangerous Goods > 5L for liquids or > 5kg for solids
REGULATORY INFORMATIONThis safety datasheet complies with the requirements of Regulation (EC) No. 1907/2006.
15.1 Safety, health and environmental regulations/legislation specific for the substance or mixture
Authorisations and/or restrictions on use
C.I. Basic violet 3 CAS-No.: 548-62-9
REACH - Candidate List of Substances of Very High Concern for Authorisation (Article 59).
Carcinogenic (article 57a)
15.2 Chemical Safety Assessment
For this product a chemical safety assessment was not carried out
OTHER INFORMATIONThe above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. The information in this document is based on the present state of our knowledge and is applicable to the product with regard to appropriate safety precautions. It does not represent any
guarantee of the properties of the product. For lab use only!